Investigation strategy for RhD typing discrepancies using a combination of PCR-SSP and serological techniques
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Investigation of Paternal RhD Zygosity by Two Molecular Methods among Blood Donors in Kurdistan Province, Iran
Background: RhD antigen system is the leading cause of hemolytic disease of the fetus and newborn (HDFN). Paternal molecular RhD zygosity test is valuable to decide on the use of anti-D immunoglobulin prophylaxis in Rh D-negative pregnant women. We aimed to investigate the paternal RhD zygosity by two molecular methods among blood donors in Kurdistan province, the west of Iran. We also compared...
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AIMS To determine whether the polymerase chain reaction with sequence specific primers (PCR-SSP) can assign HLA-DR type more accurately than serology in a routine hospital laboratory. METHODS The 93 patients currently awaiting kidney transplants have been DR typed by serology over the past 14 years, 82% within the past five years. They have now been retyped using the PCR-SSP method described ...
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BACKGROUND The knowledge of D variants in patients and donors is important because anti-D alloimmunization can occur in some but not all individuals who express a variant RHD allele. Serologic distinction of RhD discrepancies is not always straightforward, which makes molecular analysis highly desirable. METHODS A group of 223 subjects, 129 patients, and 94 blood donors was identified and ana...
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BACKGROUND AND OBJECTIVES Although widely used, serological typing of HLA loci does not always produce uequivocal results. This may be particularly the case for cord blood since samples may be of small volume and poor quality, and contaminated. DESIGN AND METHODS We typed 220 cord blood units (CBU) for HLA class I antigens using the serological technique. For those samples giving doubtful res...
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